Time-lapse 研究,囊胚皱缩与染色体非整倍性,较低的着床率和较长的卵裂时间密切相关
发布时间:2020-12-21 19:07:27

胚胎时差培养箱Embryoscope基于其强大的决策支持工具KID Score D3和KID Score D5模型,通过胚胎的形态学及形态动力学,对胚胎进行筛选评分,选择优质的胚胎进行冷冻或者移植。胚胎形态学包括多核化,细胞碎片化,细胞分裂均匀度等,其中囊胚的形态学是KID Score D5模型中的重要指标,主要通过评估囊胚的扩张程度(发育速度),卵裂形态和碎片等情况可初步筛选出相对优质的囊胚,下文即为基于囊胚的形态学(扩张程度)与其整倍性,妊娠率等的相关性研究:

Blastocyst contractions are strongly related with aneuploidy, lower implantation rates and slower embryo cleavage timing: time lapse study

 E Gazzo1, F Pe?a1, F Valdéz2, A Chung1, M Velit1, M Ascenzo1, E Escudero1

1Clínica de Fertilidad INMATER, Lima, Peru. 2Genomics Peru, Lima, Peru.

Objectives: We set out to identify contraction (CT) patterns in human blastocyst and their correlation with their ploidy status (PGT-A analysis), the time they took to reach blastocyst and the pregnancy rate.

Methods: Design: Retrospective study. 912 embryos from 270 patients that went through our center were evaluated. All embryos were cultivated in the Embryoscope incubator. From 912 embryos that started the cohort, 778 were tested for aneuploidy using an NGS platform in a reference laboratory. Blastocyst contractions (CT) were evaluated using the "embryo drawing tool" to obtain the CT percentage. 182 SET were performed, and the mean patient age was 30.44 years, ranging from 24 to 39 years.

Results: Embryos was divided in two groups, those that contracted (CT) and those that did not, we call them "solo expanding" (SE). When this was compared to ploidy status the embryos that SE showed 58.33% to be euploid while 53.58% of embryos that CT where aneuploid. This was statistically significant (p=0.029) showing that embryos that don't contract SE have higher chances of being euploid that embryos that contract CT. When we compared pregnancy rates, we also saw an increase in embryos that SE (63.10%) with embryos that CT (46.67%), this was also statistically significant (p=0.012). Finally, we saw that embryos that CT take longer to reach blastocyst stage compared to SE embryos. This also was significant (p=0.004). Finally we evaluated if the patients age an impact on whether the embryo was CT or SE and found no difference, showing that age should not be a factor y embryo contraction.

Conclusion: The results in this study show statistically significance in two analyzed traits. We showed that CT embryos show a higher chance of being aneuploid, and that they have a lower implantation rate. Also, we showed that embryos that CT take longer to reach blastocyst stage. All this was showed to happen regardless of maternal age. This proves that looking for embryo contractions could be helpful for selecting an embryo for transfer.

 摘要:为了研究分析胚胎的皱缩(CT)状态及其与染色体倍性状态(PGT-A)、达到囊胚所需的时间和妊娠率相关性?;毓诵匝芯?,评估了来自生殖中心的270名患者的912个胚胎。所有胚胎均在Embryoscope时差培养箱中培养。在该研究组的912个胚胎中,有778个胚胎使用NGS技术进行非整倍性检测。使用“胚胎绘图工具”评估囊胚皱缩(CT),以获得胚胎皱缩百分比。评估了182组样本,患者平均年龄为30.44岁,患者年龄在24至39岁之间。将胚胎分为两组,皱缩组(CT)和未皱缩组(SE,自由扩张组)。将其与倍性状态进行比较,发现SE组胚胎中为整倍体的占58.33%,而CT组胚胎为非整倍体的占53.58%,p=0.029,两者有明显的的差异,表明未皱缩组(SE)胚胎比皱缩(CT)组胚胎有更高的机会成为整倍体。当比较两组的妊娠率时,也发现SE组胚胎(63.10%)相对CT组胚胎(46.67%)有较大的增长,p=0.012两者具有明显的差异。同时,相对于SE组胚胎,CT组胚胎需要更长的时间才能到达囊胚期,p=0.004也具有显著的差异。最后,评估了患者的年龄对胚胎皱缩或者自由扩张的影响差异,但没有发现差异,表明年龄不是影响胚胎皱缩的因素。本研究的结果在两个分析的性状上具有统计学意义,发现CT胚胎显示出非整倍体的可能性更高,并且它们的妊娠率较低。此外,显示CT组胚胎到达囊胚期所需的时间更长。实验结果表明,这一切未显示与孕妇年龄相关。因此观察胚胎皱缩可能有助于选择需要移植的胚胎。


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